Device
Part:BBa_K4062024:Design
Designed by: Benjamin Bateman Group: iGEM21_WLC-Milwaukee (2021-10-16)
lactose regulated bla, lacP deletion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2913
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1981
Design Notes
lacP is inactivated by a deletion. Any gene under its control will not be expressed.
Source
Sequences from the Escherichia coli K12 genomic library from biocyc.org